Fig 5. Fitness of SkitoSnack-raised mosquito males compared to bovine blood-raised males.

(A) Survival curve of mosquito males from SkitoSnack culture and bovine blood culture with and without radiation treatment. The grey box marks the first fourteen days after the start of the experiment that were analyzed as an individual dataset. (B) Experimental setup scheme for the mating competition assay. To avoid a labeling bias, males from both diet groups, SkitoSnack (SS), and bovine blood (BB) were labeled with each stable isotope. On the right side, the three different outcomes we observed are shown. Half of the matings were set up as shown in the upper panel, the other half were set up as shown in the lower panel. (C) Paternity test results. Shown is the number of females that mated with a male from a specific diet group and the number of females that did not mate. No double matings were observed.