Figure 2. Effects of Precedent MH on Hippocampal CA1 Nerve Cell Glycolytic Pathway, Tricarboxylic Acid Cycle, and Respiratory Chain Marker Protein Expression in SH Male versus SH Female Rats.

For each treatment group, triplicate CA1 lysate pools were created for Western blot analysis of ATP-regulated kinase phosphofructokinase (PKFL; Figure 2A: male data at left, female data at right), pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1 (PDK1; Figure 2B), isocitrate dehydrogenase (IDH2; Figure 2C), or ATP synthase (ATPs; Figure 2D). Data illustrate mean normalized protein O.D. measures ± S.E.M. for male and female V¹⁻⁴ (solid white bars), V¹⁻³/SH⁴ (solid gray bars), and MH¹⁻³/SH⁴ (cross-hatched gray bars) treatment groups. *p<0.05; **p<0.01; ***p<0.001.