. 2020 Jul 3;7(5):2305–2317. doi: 10.1002/ehf2.12767

Table 2.

(A) Antibodies used for immunohistochemical staining of human myocarditis and control biopsies and (B) their respective results in mean values ± standard error of the mean

(A)
Antibody	Clone	Against	Host	Ig‐type	Dilution	Source	Specificity
CD 68	PG‐M1	Human	Mouse	IgG3	I:500	DAKO	Macrophages
CD 69	FN50	Human	Mouse	IgG1	I:50	Acris	Activated T cells
CD 209 (DC‐sign)	DCN46	Human	Mouse	IgG2b	I:50	BD Pharmingen	Immature mDCs
CD 304 (BDCA‐4/neutropilin‐1)	AD5‐17F6	Human	Mouse	IgG1	I:100	Miltenyi Biotec	pDCs
CD 123	6H6	Human	Mouse	IgG1	I:100	Serotec	pDCs
Fascin	55 K‐2	Human	Mouse	IgG1	I:100	DAKO	mDCs
(B)
Epitome (target cells)			Cells/0.2 mm² area				P value
Epitome (target cells)			Myocarditis		Controls
Fascin (mDCs)			82 ± 11.8		94.8 ± 19		n.s.
CD 209 (mDCs)			30.4 ± 2.8		33.7 ± 4.6		n.s.
CD 304 (pDCs)			76.1 ± 6.8		43.2 ± 10.5		0.011
CD 123 (pDCs)			62.2 ± 7.1		26.2 ± 5		0.001
CD 69 (activated T cells)			10.1 ± 2.7		3.1 ± 0.6		0.025
CD 68 (macrophages)			13.5 ± 2.6		6.2 ± 1.9		0.036

P‐values indicate significant differences (Student's t‐test) between myocarditis and controls. MDCs, myeloid dendritic cells; pDCs, plasmacytoid dendritic cells.