Figure 4.
Expression of bacterial cell surface protein genes during stress treatments.
JOL 401, JOL 909 and JOL 909::lon were subjected to abiotic (oxidative-1 mM H2O2; acidic-3.0 pH;osmotic- 5% NaCl and cold-4ºC) stress treatment for 5 h. The regulation of the ompF, ompD, fepA, and csgB were evaluated post-stress treatments. The results are expressed as mean fold change in the transcription levels of ompF, ompD, fepA, and csgB genes compared to unstimulated bacterial cells. The data was represented as relative mean fold change ± the SD from at least three independent experiments performed in duplicates. Means were compared by Tukey’s multiple comparison test. Similar lowercase letters indicate the non-significant difference of means (p > .05). Significant difference (*p < .05, **p < .01, ***p < .0001) compared to the wild type control. ompD- Outer membrane protein D; ompF- Outer membrane protein F; fepA- Ferric Enterobactin Transporter; csgB- Minor Curlin subunit.