Figure 9.

The kinetics of cytokine gene expression in the phagocytic and non-phagocytic epithelial cells.

The IL-2, IL-4, IFN-γ, and IL-10 mRNA expression in JOL 401, JOL 909, and JOL 909::lon infected HeLa, HepG2, and RAW cells were evaluated at various time points post-infection. The data was represented as relative mean fold change ± the SD from at least three independent experiments performed in duplicates. The experiment was performed using GAPDH as the endogenous control. IL- Interleukin; IFN- Interferon. Means were compared by Tukey’s multiple comparison test. Similar lowercase letters indicate the non-significant difference of means (p > .05). Significant difference (*p < .05, **p < .01, ***p < .001) compared to the wild-type control.