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. 2020 Jun 21;11(6):1695–1712. doi: 10.1080/19490976.2020.1777923

Table 1.

List of bacterial strains and plasmids used in this study.

Bacteria/Plasmid Genotypic characteristics Reference
S.Typhimurium    
JOL 401 Salmonella Typhimurium wild type, SPI-1 invAE+ hilA+ avr+; SPI-2, amino acid permease; SPI-3, mgtC+; SPI4, ABC transporter; SPI5, pipB+ Lab stock
JOL 909 JOL 401 ∆lon Lab stock
JOL 909::lon JOL 909 carrying pWSK29+ lon gene This study
JOL 2472 JOL 401 ∆fepA:: cat This study
JOL 2473 JOL 909 ∆fepA:: cat This study
E. coli    
DH5α E.coli FΦ80dlacZΔM15Δ (lacZYA-argF)U169 recA1 endA1 hsdR17(rk, mk+) phoA supE44 thi-1 gyr A96 relA1λ Invitrogen
Plasmid    
pWSK29 Low copy cloning vector, ApR 42
pKD46 oriR101-repA101ts; encodes lambda red genes (exo,
bet, gam); native terminator (tL3); arabinose-inducible promoter for expression (ParaB); bla
57
pKD3 oriR6Kgamma, bla (ampR), rgnB (Ter), catR, FRT 57
pHLJ 65:: GFP asd+, pBR ori,-lactamase signal sequence-based periplasmic secretion plasmid, 6 His tag containing GFP Lab stock