Table 1.
Bacteria/Plasmid | Genotypic characteristics | Reference |
---|---|---|
S.Typhimurium | ||
JOL 401 | Salmonella Typhimurium wild type, SPI-1 invAE+ hilA+ avr+; SPI-2, amino acid permease; SPI-3, mgtC+; SPI4, ABC transporter; SPI5, pipB+ | Lab stock |
JOL 909 | JOL 401 ∆lon | Lab stock |
JOL 909::lon | JOL 909 carrying pWSK29+ lon gene | This study |
JOL 2472 | JOL 401 ∆fepA:: cat | This study |
JOL 2473 | JOL 909 ∆fepA:: cat | This study |
E. coli | ||
DH5α | E.coli F−Φ80dlacZΔM15Δ (lacZYA-argF)U169 recA1 endA1 hsdR17(rk−, mk+) phoA supE44 thi-1 gyr A96 relA1λ− | Invitrogen |
Plasmid | ||
pWSK29 | Low copy cloning vector, ApR | 42 |
pKD46 | oriR101-repA101ts; encodes lambda red genes (exo, bet, gam); native terminator (tL3); arabinose-inducible promoter for expression (ParaB); bla |
57 |
pKD3 | oriR6Kgamma, bla (ampR), rgnB (Ter), catR, FRT | 57 |
pHLJ 65:: GFP | asd+, pBR ori,-lactamase signal sequence-based periplasmic secretion plasmid, 6 His tag containing GFP | Lab stock |