Table 2.
List of primers used in this study.
| Gene | Sequence (5ʹ-3ʹ) | Reference |
|---|---|---|
| catR | F:ACTTCCCGTTGCCAGCGCCTCTTTCAT TATAACCCTGTGTTTATTATGAAGTGTAGG CTGGAGCTGCTTC R:AGCATCGTTTTTGCCAATTCCCTCCC CGAATGAGGGAGGGAAGGTTGCCAATG GGAATTAGCCATGGTCC |
This study |
| FepA-F1 | F:ACTTCCCGTTGCCAGCGCCTCTTTC R:AGCATCGTTTTTGCCAATTCCCTCC |
This study |
| FepA-I2 | F:GACGGTAAACCCGTCACCAG R:ACTCCCGGTACGTTCAGACT |
This study |
| invF | F:GCAGCAAATTATTACGCCTTC R: AGTCTTCTCCCAGCATTCTC |
This study |
| hilC | F:GCAATAATCACCTTCAACAGCC R:GCCCTTCTATTTCGCTCATAC |
This study |
| sifA | F:ATTACCACCACCGCATACC R:GCTTCGACTATCTCAGCTAAC |
This study |
| sseJ | F:TCAATAAATCACATCCCAAGCC R:CCCTTCAGCAAAATTAAGCATC |
This study |
| ompD | F:GCAACCGTACTGAAAGCCAGGG R:GCCAAAGAAGTCAGTGTTACGGT |
56 |
| ompF | F:CGTGCTGGCGGTTTGTTGAC R:TTGCTGTACGCTGCGGTGAC |
56 |
| fepA | F:TTTTGCTCCGCTTCAGTC R: GTTACGCCATTATTCCAGCC |
This study |
| csgB | F:AGGAAATAATCGGGCGAAAG R:GCGAATAGCCATATGCGAC |
This study |
| sitA | F:AAAGCACCATATTCCCGCC R:AATCGACATACAGCACGCC |
This study |
| dps | F:ACCACGCAAGTTATCAACAG R:CGAACATCATTAGCGACAACAG |
This study |
|
Fur regulon |
F:CAGGAACCAGATAACCATCAC R:CCGCAATCAAGGCAGATAAG |
This study |
| corA | F:GCATATCCACTCCTTCTTCTTC R:TTTCATCCGCCAACTGTTC |
This study |
| mgtB | F:ACTGCTTTTTGTCGCCGCC R:TACCGTTGTTGCCGTGTTCC |
This study |
| mgtA | F:AACAAATTCAAAAGCCGCAG R:CAGTAATCCCACCACAAACC |
This study |
| lon | F:CGCTGAAACTGGCGGATAAA R:TAGTACTCGCGCTGGGATTT |
This study |
| Lon-C3 | F:attGAATTCTTGACCATTACGAAACTTGC R:tacAAGCTTGTTCGGAATAAAAGCGTT |
This study |
| rrsG | F:GTTACCCGCAGAAGAAGCAC R:CACATCCGACTTGACAGACC |
56 |
| IL-2 | F:TTCAAGCCCCACTTCAAGCC R:TGAGTCAAATCCAGAACATGCC |
This study |
| IL-4 | F:ACGGATGCGACAAAAATCAC R:ACCTTGGAAGCCCTACAGAC |
This study |
| IFN-γ | F:AGACAATGAACGCTACACAC R:TCTTTTCTTCCACATCTATGCC |
This study |
| IL-10 | F:GACAACATACTGCTAACCGAC R:ATCACTCTTCACCTGCTCC |
This study |
| GAPDH | F:AGAGGAGAAAGTGGGGAAAAG R:AAATCCGTTCACACCGACC |
This study |
1-primers for the flanking region of fepA;2- primers for the internal gene region of fepA;3-primers used to amplify lon for complementation experiment