Figure 6.

Microbiota composition and diversity of the male mouse offspring affected by maternal different calcium intake during pregnancy and lactation. Bacterial communities at the phylum and genus levels based on weighted UniFrac distance were shown using the PCA analysis (a and b). Estimate of bacterial richness was assessed by Chao index at the genus level (d). Estimates of bacterial diversity were assessed by Shannon and Simpson indexes of genus level (e and f). Different fecal microbiota compositions were, respectively, shown at the levels of the phylum (c) and genus (g, h). Data are presented as median and quartile, n = 8 mice/group. The differences of the data among the different calcium intake groups during the pregnancy and lactation were assessed using Mann–Whitney U-test and Wilcoxon signed-rank test. *Compared to the NC-C group, P < .05; ^#compared to the NC-HFD group, P < .05.