Figure 3.

Role of MRP5 in the regulation of ST-stimulated cGMP compartmentalization in HEMs. (a) MRP5 KD efficiency screening by quantitative RT-PCR in three different shRNA clones. Clone 192 was used for all subsequent experiments. (b) cGMP quantitation in apical, intracellular, and basolateral pools from ST-exposed HEMs (1 nM ST, 1 h) in the presence of the general PDE inhibitor IBMX (1 mM). Scr, scrambled shRNA enteroid line; MRP5 KD, MRP5 shRNA-expressing enteroid line. MK-571 (50 μM) was included in designated samples. Data points represent technical replicates from three independent experiments and graph bars are mean ± SEM. Statistical significance was determined by ANOVA. *p < .05; ***p < .001.