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. 2020 May 7;12(1):1752125. doi: 10.1080/19490976.2020.1752125

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — cGMP production and distribution differ in ST-stimulated human cancer-derived and non-cancerous primary intestinal epithelial cell lines. Measurement of cGMP in apical, intracellular, and basolateral pools obtained from human enteroid (HEM), human colonoid (HCM), Caco-2, and T84 monolayers exposed to ST (1 nM, 1 h) in the presence of IBMX (1 mM). Data points are technical replicates from three independent experiments and graph bars represent the mean ± SEM. Statistical significance was determined by ANOVA. *p < .05; **p < .01; ***p < .001.