Figure 2.

CEACAM1 deficiency impairs the intestinal permeability. (a) Naïve Ceacam1^−/− and WT mice were orally administrated with FITC-labeled dextran beads. Serum was collected five hours later, and fluorescence intensity was determined (n = 7). (b) Ceacam1^−/− and WT mice were orally gavaged with ~ 2 × 10⁹ CFU of C. rodentium. At day 10 post infection FITC-labeled dextran beads were orally administrated, and the fluorescence intensity was determined 5 h later. (c) Mice were orally gavaged with PBS or ~ 2 × 10⁹ CFU of C. rodentium. Bacterial load (CFU/g) in the liver 10 days post infection. (d) Concentration of the cytokines IL-6, IFNγ and TNFα were measured in the sera of Ceacam1^−/− and WT mice 10 dpi using Luminex technologies. All data are presented as mean ± SEM. Statistics were performed using the Student’s t-test (*, p < .05; **, p < .01).