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. 2019 Oct 4;12(1):1667723. doi: 10.1080/19490976.2019.1667723

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 5. — IFNγ production was not mediated by Group 1 ILC direct recognition of Gram-negative bacteria. (a) Percentages of LPMCs stained ex vivo for TLR2, TLR4, or TLR5 expression and gated on NK cells or ILC1s, or Lineage-positive cells that are larger on Forward Scatter-area vs Side Scatter-area flow plots.N = 6. (b) Quantification of IFNγ (pg/mL) in the supernatant of purified NK cells or (c) ILC1s exposed to recombinant IL-12p70 and IL-18 (50ng/mL), A. junii (Aj) or R. bromii (Rb) in vitro. N = 3. (d) Normalization of secreted IFNγ (pg/mL) per the amount of plated NK or ILC1s (cells/mL) to determine IFNγ (pg/cell). N = 3. Bars are mean + S.E.M. Statistical analysis performed was paired t-test or one-way ANOVA comparing the mean of each column. **p < .01, ***p < .001, n.s. = not significant.