FIGURE 4.

Schematic overview of workflow for proteomics and metabolomics. A, Proteomic workflow including homogenization (protein extraction steps 1–7), protein isolation (sample preparation steps 1‐10), protein digestion (sample preparation steps 11‐15), fractionation (sample preparation steps 16‐28), and the mass spectrometry instrument. B, Metabolomic workflow including homogenization (metabolite extraction steps 1 and 2), metal bead removal (metabolite extraction steps 3‐7), addition of internal standards (sample preparation steps 1‐10), validation run (metabolite validation steps 1‐7), and the mass spectrometry instrument