| Time interval (min) | LC solvent A (%) | LC solvent B (%) |
|---|---|---|
| 0 | 95 | 5 |
| 74 | 67.5 | 32.5 |
| 80 | 40 | 60 |
| 81 | 5 | 95 |
| 90 | 5 | 95 |
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