| Method parameter | Value |
|---|---|
| Polarity | Positive |
| Mass range (m/z) | 400‐1500 |
| Micro scans | 1 |
| Resolution | 70 000 @ 200 m/z |
| AGC target | 3E6 |
| Maximum injection time (ms) | 250 |
| dd‐MS2 | |
| Micro scans | 1 |
| Resolution | 17 500 @ 200 m/z |
| AGC targets | 2E5 |
| Maximum ion time (ms) | 64 |
| Loop count | 1 |
| Isolation window (m/z) | 2 |
| Isolation offset (m/z) | 0 |
| Fixed first mass | 100 |
| Normalized collision energy | 25 |
| Data dependent acquisition | Top12 |
| Threshold (counts) | 2E3 |
| Minimum AGC target | 3.1E4 |
| Peptide match | Preferred |
| Exclude isotopes | Enabled |
| Fragmentation type | HCD |
| Charge state rejection | Unassigned, +1, 7, >8 |
| Lock mass (445.120025 m/z) | Best |
| Dynamic exclusion (s) | 30 |
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