TABLE 2.
List of suggested reagents for use with IHC protocol
| Reagent | Supplier | Catalogue number |
|---|---|---|
| 10% neutral buffered formalin | Leica Biosystems | 3800600 |
| 4% paraformaldehyde | Fisher | 15434389 |
| EDTA | Sigma Aldrich | E9884 |
| Sub‐X | Leica Biosystems | 3803670 |
| IMS | Fisher | M/4450/17 |
| H2O2 | Sigma Aldrich | H1009 |
| Tris | Fisher | T/3710/60 |
| NaCl | Fisher | S/3120/60 |
| CaCl2 dihydrate | Honeywell | 223 506 |
| α‐chymotrypsin | Sigma Aldrich | 1002472729 |
| Goat serum | Sigma Aldrich | G9203 |
| Rabbit serum | Sigma Aldrich | R9133 |
| Donkey serum | Sigma Aldrich | D9663 |
| BSA | Sigma Aldrich | A2153 |
| Primary and secondary antibodies | See Table 3 | See Table 3 |
| VECTASTAIN Elite ABC HRP reagent | Vector Laboratories | PK‐7100 |
| DAB | Sigma Aldrich | D5905 |
| Mayer's hematoxylin | Leica Biosystems | 3801582E |
| Pertex | Leica Biosystems | 3808707E |