Table 2.
Main characteristics of the effect of FMT in the preclinical mouse model of Clostridioides difficile infection. Clinical data were measured at day 3 post-infection and biological results were measured at the time of necropsy. Clinical data are given as mean ± SEM and biological and histological data as median, 25-75% percentile. FMT-FD: CD-infected mice receiving FMT with freeze-dried microbiota; FMT-G10: CD-infected mice receiving FMT with frozen microbiota diluted in G10; FMT-G80: CD-infected mice receiving FMT with frozen microbiota diluted in G80. For each CD group treated with FMT, n = 20, i.e. 10 mice per stool sample. CD-infected mice (n = 10). All results are compared to those of untreated CD+ mice.
| FMT-FD | FMT-G10 | FMT-G80 | CD-infected mice | |
|---|---|---|---|---|
| Body weight loss vs day 0 | −4.5 ± 1.7% (p < .001) |
−11.8% ± 1.8 (NS) |
−5.1 ± 1.7 (p = .002) |
−16.0 ± 3.0 |
| Clinical score | 1.2 ± 0.3 (p < .01) |
2.5 ± 0.3 (NS) |
1.4 ± 0.3 (p < .01) |
3.0 ± 0.4 |
| CD load (log CFU/g of cecal content) |
4.9 (3.3–6.3) (p = .007) |
5.8 (4.8–6.7) (p = .06) |
4.9 (3.6–6.4) (p = .005) |
6.8 (6.3–9.9) |
| MPO (ng/g of colonic tissue) |
300 (209.3–1054) (p = .0005) |
384 ng (286.2–1529) (p = .002) |
446 (290.3–890.8) (p = .0002) |
2465 (1512–8928) |
Histopathological score
|
1.5 0–2.75 (p = .003) 1.5 0.75–4.5 (p = .005) |
4.5 3–5.75 (p = .03) 3 1.75–4.25 (p = .004) |
4.5 2.75–6.75 4.5 2–7.5 |
7.5 5.5–9 7 5.75–7.25 |