Bacterial-derived secondary bile acids are diminished upon vancomycin treatment. Bar graphs represent the serum concentration of (a) total bile acids, (b) primary unconjugated bile acids, (c) primary conjugated bile acids, (d) ratio of major primary conjugated to primary unconjugated bile acids, (e) secondary bile acids, and (f) secondary conjugated bile acids. Data are representative of six mice per group. Error bars indicate mean ± SEM. ANOVA, *p < .05. CA: cholate, α-MCA: alpha-muricholate, β-MCA: beta-muricholate, CDCA: chenodeoxycholate, TCA: taurocholate, Tα-MCA: tauro-alpha-muricholate, Tβ-MCA: tauro-beta-muricholate, TCDCA: taurochenodeoxycholate, ω-MCA: omega-muricholate, DCA: deoxycholate, LCA: lithocholate, UDCA: ursodeoxycholate, Tω-MCA: tauro-omega-muricholate, TDCA: taurodeoxycholate, TUDCA: tauroursodeoxycholate.