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Infection with rotavirus depletes mucus-filled goblet cells. Periodic Acid-Schiffs Alcian Blue (PAS-AB) stains at 1 and 3 dpi of intestinal segments (stomach, jejunum, ileum, and colon) from PBS-treated or rotavirus-infected mice. Acidic mucins are denoted by their purple staining. PAS-AB images were acquired on an upright Nikon Eclipse 90i microscope using a 20X Plan Apo (NA 0.75) DIC objective with a DS-Fi1-U2 camera and Nikon Elements software. Scale bar = 50 µm.
