Skip to main content
. 2020 Aug 17;12(1):1795388. doi: 10.1080/19490976.2020.1795388

Figure 2.

Figure 2.

Reuterin induces reactive oxygen species (ROS) in C. difficile. A. ROS production by CD2015 after treatment with glycerol or reuterin with or without 20 µM glutathione was measured by dichlorodihydrofluorescein diacetate (DCFH-DA). B. Reuterin effects on cell viability was assessed by live/dead cell stain and confirmed by the quantification of CFUs. One-Way ANOVA, * p<0.05 (n=3 biological replicates, repeated 3 independent times). C. Reuterin-glutathione interactions were assessed by derivatization of tryptophan using a colorimetric assay. Data is represented by OD560nm values (n=3 biological replicates). D. Effects of reuteirn-induced ROS on the cell membrane of CD2015 was measured by quantitation of cell membrane staining with or without 20 μM glutathione. Images were acquired at 100x on a Nikon Eclipse 90i. E. Effects of reuterin-induced ROS on DNA damage in CD2015 was evaluated by TUNEL staining with or without 20 μM glutathione and quantified using Image J (n=3 biological replicates). F. Mass spectrometry was used to produce metabolomics data from supernatants of CD2015 cultures treated with sublethal concentration of reuterin or glycerol and are represented as principle component analysis plots (n=3 biological replicates).