Figure 5.

Reuterin does not negatively influence Human Intestinal Enteroid (HIE) architecture or cell viability. A.HIEs derived from the jejunum of healthy adults were grown as 2D monolayers and differentiated in CELLVIEW slides. Monolayer integrity and cell architecture was examined by light microscopy after 16 hr of incubation with glycerol or reuterin (scale bar =100 µm). B. To assess HIE cell viability, monolayers were incubated for with resazurin and fluorescence measured from the resulting supernatant. C. HIE cell viability was confirmed through trypan blue staining of dissociated monolayers (n=3, repeated 4 independent times).