Skip to main content
. 2020 Jul 14;12(1):1785803. doi: 10.1080/19490976.2020.1785803

Figure 2.

Figure 2.

Immune response of the mouse colon by treatment of AOM/DSS and administration of LG, CT, and FCT. (a) mRNA quantification of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) and anti-inflammatory cytokines (IL-4 and IL-10) using Real-Time RT-PCR. (b) Determination of protein productions of the inflammatory cytokines using ELISA. (c) mRNA quantification of iNOS and COX-2 using Real-Time RT-PCR. (d) Determination of protein productions of iNOS and COX-2 using Western blot analysis. The mRNA level was normalized with an mRNA level of GAPDH, and the protein production was normalized with β-actin. The data present the mean ± standard deviation (SD). Asterisks denote significance vs. AOM/DSS group by one-way ANOVA (*p < .05, **p < .01, ***p < .001). The abbreviations of Con, AOM/DSS, LG, CT, and FCT represent the control mice, AOM-DSS-induced CAC mice, L. gasseri 505, C. tricuspidata leaf extract, and fermented CT by L. gasseri 505, respectively.