Figure 3.

Microbiota reposition reversed the resistance of GF mice to 5-FU-induced small intestine injury and inflammation. GF mice were conventionalized with feces from CV mice (CV→GF) for 30 d and treated with 5-FU chemotherapy or saline. CV→GF mice treated with 5-FU showed increase in clinical score (a) shortening of the intestines (b), activity of EPO (c) and MPO (d) enzymes and in the concentration of CXCL1 (e). Histopathological score (f) of the gut of control or chemotherapy-treated CV→GF and GF mice. There was increased histopathological score in CV→GF mice after 5-FU treatment compared to control and GF-5-FU groups. These data were displayed in histological representation (10X) (g). The data represent the mean ± SEM of six animals per group. *p < 0.05 vs respective control, #p < 0.05 vs GF-5-FU.