Figure 2.

Acute fasting induces villus atrophy and reduced proliferation in the jejunum. Intestinal architecture and proliferation were assessed using H&E staining and Ki67 immunohistochemistry, respectively, in N = 6 animals. Fasting decreases villus length (**P = .0054; A/B) yet had no effect on crypt depth (c). The number of Ki67+ cells in the jejunum decreased following 48-h fasting (*P = .01; D). Data shown as individual points and mean. All data were analyzed with an unpaired t-test, where P < .05 was considered significant.