Figure 5.

Deletion of flgM gene reduces LF82 adhesion to and invasion of intestinal epithelial Caco-2/TC7 cells but increases their interleukin-8 response, A: The numbers of cell-associated bacteria were quantified after a 3-h infection period. The results are expressed as the percentage of adherent wild-type LF82 bacteria. B: Invasion ability was determined after gentamicin treatment for an additional hour. The results are expressed as the percentage of invasive wild-type LF82 bacteria taken as 100%. C: Representative western blot analysis of flagellin release in the culture supernatant at 24h. Quantification of FliC band intensity standardize to total protein is shown in the top panel (representative from 4 independent experiments). D: Pro-inflammatory chemokine interleukin-8 secretion by Caco-2/TC7 cells after a 3-h infection. The data are presented as the mean ± SEM of at least four independent experiments Statistical analysis were performed using one-way ANOVA with Tukey's multiple comparison test. P <  0.05 (*),P <  0.01 (**) and P <  0.005 (***).