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. 2020 Jan 15;11(4):771–788. doi: 10.1080/19490976.2019.1707015

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Lactobacillus plantarum (LP) and Pediococcus pentosaceus (PP) induce responses associated with IFN-I production in monocytes and macrophages from PBMCs. (a) Production of IFN-β in supernatants obtained from primary macrophages after 0, 4, 8 and 12 h of being exposed to LP and PP. The INF-β levels were calculated using the Human IFN-β Quantikine ELISA Kit. Data are representative of three healthy donors and are mean with SD from two biological replicates. (b-c) Expression of (b) CD64 and (c) CD40 in monocytes (blue) and neutrophils (red) exposed to LP and PP as well as L. casei (LC) as a control (no IFN-I inducer). The CD expression is presented as a percentage of fold change (increase or decrease) over a non-stimulated condition using unchallenged monocytes and neutrophils. Data are mean with SD from two healthy donors and the comparative analysis was carried out with two-way ANOVA and Tukey multiple comparison (*p < .05; **p < .01).