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. 2020 Sep 8;12(1):1813532. doi: 10.1080/19490976.2020.1813532

Figure 4.

Figure 4.

Vivomixx administration in the chronic phase of TMEV-IDD promotes anti-inflammatory responses in the spinal cord. A) Representative images of transversal spinal cord sections immunostained with Iba-1 to label microglia. Scale bar 100 μm. Below are higher magnification images (63x) of Iba-1 stained cells that indicate the changes in morphology. Scale bar 10 μm. Quantification of the area occupied by Iba-1 staining is also shown on the right. B) Representative images of GFAP (red) and Vimentin (green) staining in the spinal cord together the quantification of Vimentin-GFAP overlap through the Manders coefficient measure. C) qRT-PCR analysis of inflammatory markers (mIL-1β, mIL-6, mTNF-α, mIL-4 or mIL-10) in the spinal cord as assessed using the 2−ΔΔCt method. *p < .05 vs. S; **p < .01 vs. S; ***p < .001 vs. S; #p < .05 vs. T; ##p < .01 vs. T.