Figure 1. Experimental design and mitochondrial function.

(A) IH paradigm with experimental outcomes. (B) Representative tracing of mitochondrial respiration. (C) Mitochondrial states 3 and 4 respiration rates (unpaired t test). (D) Respiratory control ratio (RCR) and ADP/O (nmol of phosphorylated ADP per atom of the O₂) ratio (Mann-Whitney U test). (E) Cerebral ATP content at the end of 10 IH events (unpaired t test). (F) Immunostaining for TUNEL⁺ cells in normoxic and IH P10 mouse brains. Scale bar: 100 μm. (G) Confocal microscopy of external capsule stained for CC1, Olig2, MBP, NG2, and DAPI with CC1⁺Olig2⁺ cell count. Dashed outline is the area selected for assessment of CC1⁺Olig2⁺ cell density, expressed as a percentage of total Dapi⁺ cells. Scale bar: 100 μm.