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. 2020 Aug 31;130(10):5302–5312. doi: 10.1172/JCI133780

Figure 4. Premature termination renders the TFshort mRNA a substrate for nonsense-mediated decay.

Figure 4

(A) Organization of the exons contributing to the canonical F3 transcript highlights the site of premature termination (Ter). Splicing schematic generated using Ensembl (47). (B and C) F3 mRNA expression was evaluated by quantitative PCR in ECs (B, n = 4) and VSMCs (C, n = 3) derived from each BLK or HOM clone following 3 hours treatment with 100 μM cycloheximide (CHX) or vehicle. Relative mRNA expression for F3 (CHX vs. vehicle treatment) is presented as mean ± SEM. Unpaired t test. *P < 0.05, **P < 0.01.