Skip to main content
. 2020 Aug 31;130(10):5302–5312. doi: 10.1172/JCI133780

Figure 5. Impaired coagulation initiation and hemostasis in F3+/– mice replicating heterozygous TF deficiency.

Figure 5

Primary ear fibroblasts were cultured from F3+/– and F3+/+ littermate controls and assayed for their ability to support the factor VIIa-mediated conversion of factor X to factor Xa, as determined using a chromogenic factor Xa substrate that absorbs at 405 nm. A negative control omits factor VIIa (-VIIa). (A) Representative kinetic course plots the mean ± SEM of 3 replicates. (B) Factor Xa generation (nM/min) across n = 5 independent experiments was quantified; 1-way analysis of variance. (C) Eight- to 10-week-old F3+/– and F3+/+ littermate controls were evaluated in an aggressive tail amputation model and time to initial cessation of bleeding was recorded over 30 minutes of observation for n ≥ 14 mice of each genotype. Unpaired t test. (D) Kaplan-Meier cumulative survival of mice in C following tail transection. Log rank test. *P < 0.05.