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. 2020 Sep 29;158:309–320. doi: 10.1016/j.theriogenology.2020.09.022

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Fig. 3 — AA2P and global methylation level of nucleic acids. (A, C) Immunofluorescence images of 5 mC and m⁶A staining in porcine iSCs treated with or without AA2P (250 μM) for 36 h. Green, 5 mC and m⁶A; Blue, Hoechst33342. Scale bar: 50 μm for 5 mC staining and 25 μm for m⁶A staining. (B, D) Relative fluorescence intensity analysis of 5 mC and m⁶A levels. Abbreviations:5 mC, N5-methylcytidine; m⁶A, N6-methyladenosine. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)