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. 2020 Sep 29;9:e60066. doi: 10.7554/eLife.60066

Figure 3. Dermal Lef1 is required to support hair follicle regeneration in neonatal wounds.

Figure 3.

(a) Schematic representation of transgenic strategy to ablate Lef1 expression in dermal fibroblasts. Scale bars are 50 μm (b–e) Verifying dermal Lef1 ablation by immunostaining. White arrows indicate papillary regions (b–c) or dermal papilla (d–e). Scale bars are 50 μm (f) Schematic describing 2 mm circular wounds and harvest times to test if dermal Lef1 is required for regeneration. (g–i) Immunostaining of P2 7 days post wound (7dpw) skin for Itga6 and Lef1. (g-h Scale bars are 100 μm) Wound beds are highlighted by white outline. White arrows indicate regenerating hair follicles. (i-j Scale bars are 50 μm) (k) Quantification of regenerating hair follicles in wounds beds of n = 4 Lef1fl/fl (WT) and n = 3 Twist-Lef1cKO mice. p<0.003.