Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Clytia hemisphaerica) | CheNanos1 | GenBank | JQ397274 | |
| Gene (Clytia hemisphaerica) | CheWnt3 | GenBank | EU374721 | |
| Gene (Clytia hemisphaerica) | CheWnt6 | GenBank | EU374719 | Named CheWnt6 following Condamine et al., 2019 |
| Gene (Clytia hemisphaerica) | CheAxin | GenBank | EU374716 | |
| Gene (Clytia hemisphaerica) | Cheβ-cat | GenBank | JQ438997 | |
| Gene (Clytia hemisphaerica) | CheBra1 | GenBank | DQ872898 | |
| Gene (Clytia hemisphaerica) | CheFoxA | GenBank | GBGP01000196 | |
| Gene (Clytia hemisphaerica) | Z4B strain | Leclère et al., 2019 | Polyp colony producing female jellyfish | |
| Antibody | Rhodamine goat anti-rabbit secondary antibody | Jackson ImmunoResearch | IF (1:500) | |
| Antibody | Alexa Fluor 488 or 594 goat anti-rat secondary antibodies | Thermo Fisher Scientific | #A-11006 #A-11007 |
IF (1:200) |
| Antibody | anti-Myosin Light Chain two phosphorylated at serine19 (PMyo) rabbit polyclonal | Cell signaling technology | #3671 | IF (1:200) |
| Antibody | anti-tyrosinated tubulin antibody (YL1/2) rat monoclonal | Abcam | #6160 | IF (1:50) |
| Chemical compound, drug | Hydroxyurea | Sigma-Aldrich | #H8627 | |
| Chemical compound, drug | Blebbistatin | Sigma-Aldrich | #B0560 | |
| Chemical compound, drug | BDM (2,3-Butanedione monoxime) | Sigma-Aldrich | #B0753 | |
| Chemical compound, drug | Menthol | Sigma-Aldrich | #M2772 | |
| Chemical compound, drug | PKF118-310 | Sigma-Aldrich | #K4394 | |
| Commercial assay, kit | EdU Click-It kit (Alexa Fluor 555) |
Thermo Fisher Scientific | #C10338 | |
| Other | FluoSpheres | Molecular Probes | #F8812 | |
| Other | DiI | Thermo Fisher Scientific | #D3911 | |
| Other | Alexa Fluor 488 Phalloidin | Thermo Fisher Scientific | #10125092 | (1:100) |
| Other | Hoechst dye | Sigma-Aldrich | #94403 | (1 µg/ml) |
| Other | Reference transcriptome (Clytia hemisphaerica) |
Leclère et al., 2019 | ENA identifier: PRJEB28006 | Genome guided transcriptome |
| Other | RNAseq samples (Clytia hemisphaerica) |
This study | ENA identifier: PRJEB37920 | BGISEQ-500 50nt single reads generated for 12 RNA samples:pooled samples of umbrella central area collected at six time points during manubrium regeneration (two biological replicates for each). |