Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Danio rerio) | Tg(kdrl:EGFP)s843 | Jin et al., 2005 | ZFIN: s843 | |
| Genetic reagent (D. rerio) | Tg(kdrl:TagBFP)mu293 | Matsuoka et al., 2016 | ZFIN: mu293 | |
| Genetic reagent (D. rerio) | Tg(kdrl:dsRed2)pd27 | Kikuchi et al., 2011 | ZFIN: pd27 | |
| Genetic reagent (D. rerio) | Tg(fli1a:Gaf4ff)ubs4 | Zygmunt et al., 2011 | ZFIN: ubs4 | |
| Genetic reagent (D. rerio) | Tg(UAS:Kaede)rk8 | Herwig et al., 2011 | ZFIN: rk8 | |
| Genetic reagent (D. rerio) | TgBAC(pdgfrb:EGFP)ncv22 | Ando et al., 2016 | ZFIN: ncv22 | |
| Genetic reagent (D. rerio) | Tg(kdrl:NLS-mCherry)is4 | Wang et al., 2010 | ZFIN: is4 | |
| Genetic reagent (D. rerio) | Tg(12xSBE:EGFP)ia16 | Casari et al., 2014 | ZFIN: ia16 | |
| Genetic reagent (D. rerio) | TgBAC(tgfbr1b:EGFP,cryaa:CFP)bns330 | This manuscript | ZFIN: bns330 | |
| Genetic reagent (D. rerio) | Tg(fli1a:tgfbr1b-P2A-mScarlet-Hsa.HRAS)bns421 | This manuscript | ZFIN: bns421 | |
| Genetic reagent (D. rerio) | tgfbr1abns329 | This manuscript | ZFIN: bns329 | |
| Genetic reagent (D. rerio) | tgfbr1bbns225 | This manuscript | ZFIN: bns225 | |
| Antibody | Anti-GFP (chicken polyclonal) | AvesLab | Cat#: GFP-1020 | 1:400 |
| Antibody | Anti-Elastin2 (rabbit polyclonal) | Miao et al., 2007 | 1:100 | |
| Antibody | Anti-tRFP (rabbit polyclonal) | Evrogen | Cat# AB233 | 1:200 |
| Antibody | Secondaries Alexa FluorTM 488-568-647 IgG (H+L) (goat polyclonal) | Thermo Fisher Scientific | 1:500 | |
| Antibody | Anti-Smad3 (phospho S423 + S425) antibody (rabbit monoclonal) | Abcam | Cat# ab52903 | 1:100 |
| Antibody | nti-phospho-Smad1/5 (Ser463/465)/Smad9 (Ser465/467) (rabbit monoclonal) | Cell Signaling Technology | Cat# 13820 | 1:100 |
| Antibody | Anti-dsRed (rabbit polyclonal) | Takara Bio Clontech | 632496 | 1:200 |
| Antibody | Anti-Alcama/Dm-Grasp (mouse monoclonal) | DSHB | ZN-8 | 1:50 |
| Commercial assay or kit | Click-iT EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye | Thermo Fisher Scientific | Cat# C10340 | |
| Commercial assay or kit | In-Fusion HD Cloning Plus | Takara Bio | Cat# 638910 | |
| Commercial assay or kit | DyNAmo ColorFlash SYBR Green qPCR Mix | Thermo Scientific | Cat# F416S | |
| Chemical compound, drug | E-616452 | Cayman Chemical | 14794 | |
| Chemical compound, drug | SIS3 | Calbiochem/Merck | 566405 | |
| Chemical compound, drug | EdU | Thermo Fisher Scientific | Cat# A10044 | |
| Chemical compound, drug | FITC-dextran 2000 kDa | Sigma | Cat# 52471 | |
| Chemical compound, drug | LysoTracker Deep Red | Thermo Fisher Scientific |
Cat# L12492 | |
| Commercial assay or kit | miRNeasy micro Kit | Qiagen | Cat# 217084 | |
| Commercial assay or kit | mMessage mMachine T3 Transcription Kit | Thermo Fisher Scientific | Cat# AM1348 | |
| Commercial assay or kit | mMessage mMachine T7 Transcription Kit | Thermo Fisher Scientific | Cat# AM1344 | |
| Commercial assay or kit | DIG RNA labeling kit | Roche | Cat# 11277073910 | |
| Commercial assay or kit | Maxima First Strand cDNA kit | Thermo Fisher Scientific | Cat# K1641 | |
| Commercial assay or kit | MegaShortScript T7 | Thermo Fisher Scientific | Cat# AM1354 | |
| Commercial assay or kit | RNA Clean and Concentrator Kit | Zymo Research | Cat# R1013 | |
| Software, algorithm | ZEN Blue 2012 | Zeiss, Germany | ||
| Software, algorithm | ZEN Black 2012 | Zeiss, Germany | ||
| Software, algorithm | Imaris - Version 8.4.0 | Bitplane, UK | ||
| Software, algorithm | GraphPad Prism 6 | GraphPad Software, USA | ||
| Software, algorithm | FIJI/ImageJ | Schindelin et al., 2012 |