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. Author manuscript; available in PMC: 2020 Sep 29.
Published in final edited form as: Vaccine. 2020 Jul 25;38(37):5885–5890. doi: 10.1016/j.vaccine.2020.07.008

Fig. 2. Mouse vaccination with high ADCC activating peptides results in partial protection from influenza infection.

Fig. 2.

(A) Serum ADCC activity (by NFAT signaling of luciferase reporter NK cells) to H1N1-infected target cells 21 days post-vaccine. Day 14 wt loss (B) and survival (C) of vaccinated mice after lethal H1N1 challenge. TCID50 lung viral titers (D) and fold reduction of lung viral loads (E) at day 7 versus scrambled vaccinated mice. NK cell response in lungs at day 7 post infection for total NK cells (F), CD69+ NK cells (G), IFN-γ+ NK cells (H) and CD107a+ NK cells (I) (see gating FACS plots in Supplementary Fig. 2C). Data represents the mean average and SD. For (A) survival curves were compared for statistical significance with Log-rank Mantel-Cox test (n = 5 per group). For (B–I) data were compared for statistical significance by one-way ANOVA with Dunnett’s multiple comparison test versus scrambled or PBS group, (n = 3 per group) *p < 0.05, **p < 0.01, ***p < 0.005, experiments were repeated twice