Table 1.
Target gene | Primer sequences 5′-3' | Amplicon (bp) | PCR conditions | References | ||||
---|---|---|---|---|---|---|---|---|
No of cycles | Denaturation | Annealing | Extension | Final Extension | ||||
oprL | ATG GAA ATG CTG AAA TTC GGC CTT CTT CAG CTC GAC GCG ACG | 504 | 40 | 96 ˚C for 1 min | 55 ˚C for 1 min | 72 ˚C for 1 min | 72˚C for 10 min | 34 |
toxA | GACAACGCCCTCAGCATCACCAGC CGCTGGCCCATTCGCTCCAGCGCT | 396 | 30 | 94 ˚C for 1 min | 55 ˚C for 1 min | 72 ˚C for 1 min | 37 | |
exoS | GCGAGGTCAGCAGAGTATCG TTCGGCGTCACTGTGGATGC | 118 | 36 | 94 ˚C for 30 s | 58 ˚C for 30 s | 68 ˚C for 1 min | 35 | |
phzM | ATGGAGAGCGGGATCGACAG ATGCGGGTTTCCATCGGCAG | 875 | 30 | 94 °C for 30 s | 54 ˚C for 30 s | 72 ˚C for 1 min | 36 | |
blaTEM | ATCAGCAATAAACCAGC CCCCGAAGAACGTTTTC | 516 | 32 | 94 °C for 30 s | 54 ˚C for 30 s | 72 ˚C for 1 min | 38 | |
tetA | GGTTCACTCGAACGACGTCA CTGTCCGACAAGTTGCATGA | 576 | 30 | 94 ˚C for 1 min | 56 ˚C for 30 s | 72 ˚C for 1 min | 39 | |
blaCTX-M |
ATGTGCAGYACCAGTAARGT TGGGTRAARTARGTSACCAGA |
593 | 35 | 95 ˚C for 45 s | 51 ˚C for 45 s | 72 ˚C for 1 min | 40 |