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. 2020 Sep 29;10:15961. doi: 10.1038/s41598-020-72264-4

Table 1.

List of oligonucleotide sequences and their PCR conditions used in the current study.

Target gene Primer sequences 5′-3' Amplicon (bp) PCR conditions References
No of cycles Denaturation Annealing Extension Final Extension
oprL ATG GAA ATG CTG AAA TTC GGC CTT CTT CAG CTC GAC GCG ACG 504 40 96 ˚C for 1 min 55 ˚C for 1 min 72 ˚C for 1 min 72˚C for 10 min 34
toxA GACAACGCCCTCAGCATCACCAGC CGCTGGCCCATTCGCTCCAGCGCT 396 30 94 ˚C for 1 min 55 ˚C for 1 min 72 ˚C for 1 min 37
exoS GCGAGGTCAGCAGAGTATCG TTCGGCGTCACTGTGGATGC 118 36 94 ˚C for 30 s 58 ˚C for 30 s 68 ˚C for 1 min 35
phzM ATGGAGAGCGGGATCGACAG ATGCGGGTTTCCATCGGCAG 875 30 94 °C for 30 s 54 ˚C for 30 s 72 ˚C for 1 min 36
blaTEM ATCAGCAATAAACCAGC CCCCGAAGAACGTTTTC 516 32 94 °C for 30 s 54 ˚C for 30 s 72 ˚C for 1 min 38
tetA GGTTCACTCGAACGACGTCA CTGTCCGACAAGTTGCATGA 576 30 94 ˚C for 1 min 56 ˚C for 30 s 72 ˚C for 1 min 39
blaCTX-M

ATGTGCAGYACCAGTAARGT

TGGGTRAARTARGTSACCAGA

593 35 95 ˚C for 45 s 51 ˚C for 45 s 72 ˚C for 1 min 40