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. 2020 Sep 29;3:536. doi: 10.1038/s42003-020-01268-7

Fig. 3. A conserved cysteine is involved in the redox regulation of Engrailed transfer.

Fig. 3

a Conserved cysteine residue in Engrailed 2 proteins across species (the hexapeptide is underlined). b Gel-shift assay comparing the DNA-binding properties of EN2, EN2C>S and DNA-binding deficient EN2W>K. c Quantification of EN2C>S and EN2 transcriptional activity on the MAP1b promoter. d–f Quantification of EN2 or EN2C>S secretion via the transRUSH method from control cells d or cells expressing Lck-DAO with or without d-Ala e or inactive or active Catalase (CATmut or CAT, respectively) f. Quantification of EN2 or EN2C>S internalization g after DTT pretreatment h or EN2 dimerization i. *p-value ≤ 0.05; **p-value ≤ 0.01; ***p-value ≤ 0.001; and ****p-value ≤ 0.0001.