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. 2020 Jun 17;123(7):1123–1130. doi: 10.1038/s41416-020-0940-5

Fig. 3. Direct targeting of miR-17-5p to the 3′UTR of VIM mRNA.

Fig. 3

a In LoVo and HT29 cell lines, the interaction between vimentin and miR-17-5p was assessed by RNP IP as described in the “Materials and methods” section. The expression of vimentin in the miR-17-5p IP materials was determined by RT-qPCR. b Schematic representation shows the construction of wild-type or mutant, or perfectly matched vimentin 3′UTR in the vector. c Schematic representation shows the plasmid containing the vimentin 3′UTR in luciferase assay. d Luciferase assays show a decrease in reporter activity after co-transfection with miRNA17 mimics and the wild-type vimentin 3′UTR, and the perfect match in LoVo cells. Mutant of vimentin 3′UTR did not show a decrease. The bars represent mean ± SD. **P < 0.001. All the experiments were repeated three times.