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. 2020 Jun 12;16(3):313–325. doi: 10.1007/s11302-020-09706-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Expression of mRNA for the Nlrp3 inflammasome complex in mGr1⁺BM cells after stimulation with AMD3100, G-CSF, ATP and S1P measured by qRT-PCR. Expression of Nlrp1, Nlrp3, Aim-2, Hmgb1, IL-1β, Il-18, Casp1, mRNAs in murine bone marrow Gr-1⁺cells after stimulation for 6 h, in RPMI medium plus 0.5% BSA with AMD3100, G-CSF, ATP and S1P as measured by qRT-PCR. Results of qRT-PCR were normalized to the β2 microglobulin (β2m) level. Combined data from three independent experiments is shown. *p < 0.05; **p < 0.001