Fig. 5.

Effects of soluble plasma fibronectin (pFN) and its WJ-MSC-conditioned media on the response of cultured HSC-T6 cells to TGF-β1 stimulation. A HSC-T6 cells were grown on dishes precoated with or without pFN, received 2 h of serum starvation, followed by TGF-β stimulation (10 ng/ml). Alternatively, B soluble pFN or C serum-free conditioned media of WJ-MSCs with soluble pFN were added into HSC-T6 cells 2 h before TGF-b stimulation. Western blotting and semi-quantitative densitometrical analysis were used to measure the contents of D–F cellular Smad2 phosphorylation and G–I RhoA protein. Density data are mean ± SD from three independent experiments. *p < 0.01, vs. time zero control or between indicated groups