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. 2020 Sep 10;17(5):717–727. doi: 10.1007/s13770-020-00282-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Seeding of cells. A MSCs of primary passage; B Uniform morphology of the 3rd passage MSCs; C confluent MSCs induced with osteogenic media showed intracellular refractive granules; D after 3-week induction, MSCs clused and formed calcified nodules; E calcified nodules formation from osteogenically-induced MSCs confirmed by alizarin red staining; F ALP expression of osteogenically differentiated MSCs visualized by the modified Gomori staining. Scale bar = 100 μm