Fig. 3.

Circ_0068655 acted as a sponge to negatively regulate miR-498. A Levels of circ_0068655 in the nuclear and cytoplasmic fractions in HCMs were confirmed via qRT-PCR. B Graphical representation showing the predicted sites of miR-498 for binding to circ_0068655 and its corresponding mutation. Luciferase reporter assay for the interaction between miR-498 and circ_0068655 in C HCMs and D HEK-293T cells. E The expression of miR-498 in HCMs after transfected with shRNAs against circ_0068655 or empty vector (sh-Ctrl) was determined by qRT-PCR. F The expression of miR-498 in HCMs after transfected with circ_0068655 plasmid or empty vector (Vector) was determined by qRT-PCR. The data are expressed as the mean ± SD. *p < 0.05; **p < 0.01, ns: no significant difference