Table 1.
Primer sequences in this study
| Primer | Forward primer | Reverse primer |
|---|---|---|
| U6 | AACGCTTCACGAATTTGCGT | CTCGCTTCGGCAGCACA |
| circ_00686552 | GCCCAGTACCAGTTCA | CCAGTATTTCCTGCTTT |
| miR-498 | TTTCAAGCCAGGGGGCGTTTTTC | GCTTCAAGCTCTGGAGGTGCTTTTC |
| PAWR | GCCGCAGAGTGCTTAGATGAG | GCAGATAGGAACTGCCTGGATC |
| Lentiviral-based shRNA targeting circ_0068655 sequences | ||
| circ_0068655 | ||
| shRNA #1 | ccggGTACCAGTTCAGCAAGTGTCTggatccAGACACTTGCTGAACTGGTACtttttg | aattcaaaaaGTACCAGTTCAGCAAGTGTCTggatccAGACACTTGCTGAACTGGTAC |
| shRNA#2 | ccggGCAAGTGTCTCTACTGTCAGAggatccTCTGACAGTAGAGACACTTGC tttttg | aattcaaaaaGTACCAGTTCAGCAAGTGTCTggatccAGACACTTGCTGAACTGGTAC |
| Circular RNA and the corresponding mutation of miR-498 binding site sequences | ||
| Circular RNA | ||
| circ_0068655 | ACGCTCGAGGTGCAAGTGAAAGTGT | ACGGCGGCCGCCACCAAATAATGGTTCTGGTTCCA |
| circ_0068655 binding site | TGTAGGAAAACATATGGTAGCCG | CGGCTACCATATGTTTTCCTACA |
| PAWR | ACGCTCGAGAATTTAAGGGATTTTCTTC | ACGGCGGCCGCTCCCAAAGTGCTGGGATTAC |
| PAWR binding site | TAATATATGAAAGGGAATAGCCT | AGGCTATTCCCTTTCATATATTA |