A, B Proliferation after days 7, 14, and 21 under direct condition and days 1, 3, 5, 7, and 9 under indirect conditions. C: control; BB: big size microcarrier with BMP-2; SV: small size microcarrier with VEGF; SVBB: mixed microcarrier with BMP-2 and VEGF. Asterisks represent samples presenting significantly higher cell growth (* = p < 0.05). C Contrast microscopy images obtained for cell culture under indirect culture conditions. Magnification × 100, scale bar = 300 μm