Fig. 1.

Proportions of T and B cells in freshly isolated PBMCs. a, b PBMCs, freshly isolated by Ficoll-Paque density gradient centrifugation, were labelled with fluorochrome-conjugated mAbs and data acquired by flow cytometry. a Sequential flow cytometric gates were selected left to right (top to bottom), as shown, to gate single cells, lymphocytes, and live (Zombie NIR⁻) CD45⁺ lymphocytes, in order to determine b proportions of CD3⁺CD19⁻ T cells and CD3⁻CD19⁺ B cells amongst CD45⁺ cells. Data represents group means ± SD (n = 11 donors); symbols represent individual donors