Fig. 2.

Proportions of CD39^+/−CD73^+/− T and B cell subsets in freshly isolated PBMCs. a–d PBMCs, freshly isolated by Ficoll-Paque density gradient centrifugation, were labelled with fluorochrome-conjugated mAbs and data acquired by flow cytometry. a, b Cells were gated as shown in Fig. 1, to identify a CD3⁺CD19⁻ T cells and b CD3⁻CD19⁺ B cells, with fluorescence minus one (FMO)-stained samples used to set CD39 and CD73 quadrants, to determine the proportions of c CD39^+/−CD73^+/− T cells and d CD39^+/−CD73^+/− B cells. c, d Data represents group means ± SD (n = 11 donors); symbols represent individual donors