Fig. 3.

Proportions of CD3⁺ T cells and CD19⁺ B cells are not altered by cryopreservation. a, b PBMCs, freshly isolated by Ficoll-Paque density gradient centrifugation and corresponding PBMCs cryopreserved for 1-, 3- and 6 months were labelled with fluorochrome-conjugated mAbs, and the proportions of a CD3⁺CD19⁻ T cells and b CD3⁻CD19⁺ B cells amongst CD45⁺ cells were determined by flow cytometry. a, b Connected symbols represent individual donors (n = 11); P < 0.05 compared with corresponding freshly isolated PBMCs indicates significance; ns, no significances; paired two-tailed Student’s t test