Fig. 7.

Ficoll-Paque and SepMate density gradient centrifugation yield similar viabilities and proportions of T and B cells in 1-month cryopreserved PBMCs. a–c At time of isolation, aliquots of PBMCs were cryopreserved for 1 month. Upon thawing, cell viabilities were assessed using trypan blue and a haemocytometer. b, c PBMCs were incubated with fluorochrome-conjugated mAbs and the proportions of b CD3⁺CD19⁻ T cells and c CD3⁻CD19⁺ B cells of CD45⁺ cells were determined by flow cytometry. a Bars represent group means, and a individual or b, c connected symbols represent individual donors (n = 6); P < 0.05 indicates significance; ns, no significances; paired two-tailed Student’s t test