Skip to main content
. 2020 Sep 16;10:487. doi: 10.3389/fcimb.2020.00487

Figure 2.

Figure 2

Interrogation of ABCF1 under basal conditions in HBEC-6KT in vitro. (A) Immunoblot confirming siRNA-mediated knockdown of ABCF1 protein expression in HBEC-6KT cells. (B) Quantification of ABCF1 protein expression following siRNA treatment. (C) LDH quantification as a measure of cell viability following siRNA treatment. (D) Phase-contrast microscopy (4X magnification) of HBEC-6KT following siRNA treatment. (E) PCA plot of microarray gene expression profiles of HBEC-6KT cells following siRNA treatment. Red circles (media alone), green circles (transfection reagent only), blue circles (transfection reagent and control siRNA), purple circles (transfection reagent and ABCF1 siRNA). (F) Log2 expression data for transfection reagent with ABCF1 siRNA compared to transfection reagent with control siRNA. Significantly differently expressed genes are in blue and are down-regulated (ABCF1 and C12orf75). All studies n = 3. **p < 0.01.