Figure 2.

Representative images of diffuse and localized adherence to HT-29 cells by E. coli strain 186 (a), Enterobacter spp. strain 136 (b), Citrobacter freundii strain 152 (c), and Klebsiella pneumoniae strain 195 (d). (a′) shows the DAPI channel from (a); where arrows indicate some attached bacteria. (c′) shows a detail of the boxed area in (c) and arrows indicate individual attached bacteria. In (d), a central area of the HT-29 cells is shown with the DAPI channel in the boxed area. Arrows indicate individual attached bacteria at the edge of the cells and open arrows in the boxed areas indicate some clusters of bacteria on the cell surface. Cells were stained with Atto-488 phalloidin (green) and DAPI (blue). Scale bars: (a,b) 10 μm; (c) 5 μm. Original magnification: (a,b,d) ×600; (c) ×400.